Heme

A quantitative, plate-based assay designed for measuring heme concentration in biological samples. The procedure supports direct analysis and is suitable for microplate-reader workflows, including automated laboratory setups.

Heme quantitative assay kit for microplate analysis

Product Information

Product Name Heme
Catalog Number 0986-MA-0190
Package Size 100 Well
Assay Type Quantitative
Detection Method Colorimetric microplate reader, OD 570 nm
Detection Range 5–160 pg (10–250 fmol), as specified
Reactivity All
Shipping Conditions Gel pack
Dry Ice Required No
Hazardous Product No
Availability 24/48H stock items; 2 to 6 weeks for non-stock items
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Product Description

Heme is an iron-containing porphyrin molecule that plays an essential role in oxygen transport, electron transfer, and numerous enzymatic reactions. Quantification of heme can help researchers investigate cellular metabolism, oxidative processes, blood-related biology, and other pathways involving heme-containing proteins.

This assay uses a simple, direct, plate-based procedure designed to measure heme concentration. Its microplate format supports processing multiple samples in parallel and can be integrated into laboratory workflows using a compatible plate reader.

The assay is intended for quantitative analysis in tissue extracts, urine, serum, plasma, and other biological fluids, subject to compatibility with the manufacturer's protocol.

Key Features

  • Quantitative analysis: Designed to measure heme concentration in biological samples.
  • Microplate-based format: Allows multiple samples to be analyzed in parallel.
  • Colorimetric detection: Measurement at an optical density of 570 nm.
  • Automation-ready workflow: Plate-based procedure suitable for compatible laboratory automation.
  • Multiple sample types: Intended for tissue extracts, urine, serum, plasma, and other biological fluids.

Applications

  • Heme concentration measurement
  • Research into heme metabolism and biological pathways
  • Studies involving heme-containing proteins
  • Analysis of biological extracts and fluids
  • Cellular metabolism and biochemical research
  • High-throughput sample analysis using microplate readers

Sample Types

The listed sample types include:

  • Tissue extracts
  • Urine
  • Serum
  • Plasma
  • Other biological fluids
Important: Sample preparation, compatibility, and the need for dilution depend on the sample matrix and assay instructions. Follow the manufacturer's protocol for reliable measurements.

Detection Method and Assay Specifications

Measurement Principle Colorimetric
Instrument Compatible microplate reader
Detection Wavelength 570 nm
Assay Format Plate-based
Assay Type Quantitative
Listed Detection Range 5–160 pg (10–250 fmol)
Package Size 100 Well
Catalog Number 0986-MA-0190

Confirm the applicable calibration range, sample volume, and interpretation criteria in the manufacturer's instructions before beginning an experiment.

Alternative Products

Explore related products for heme research, heme quantification, heme oxygenase studies, and associated biochemical applications.

Ordering Information

Heme, catalog number 0986-MA-0190, is supplied in a 100-well format. Log in to Gentaur to check current pricing and contact the team for availability and ordering assistance.

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For research use only. Not intended for diagnostic or therapeutic use.